Human Anti-ERBB2 Rcombinant Antibody (TP-083CL)

Customer Review

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Laura Brown
03.Dec,23

Ideal for HER2 Research

The Trastuzumab has been excellent for our HER2 research. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.

Emily Lewis
09.Aug,22

Reliable in Immunoassays

We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for HER2 is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.

John Carter
26.Sep,21

Consistent and Effective

This antibody has proven to be consistent and effective in our various assays. The high-quality, reproducible results have significantly improved our experiments, making it a crucial part of our research toolkit. The strong affinity and specificity for HER2 have been particularly beneficial.

Q&As

1. Is the Trastuzumab antibody suitable for use in flow cytometry analysis?

   A: Yes, the Trastuzumab antibody (TP-083CL) is suitable for use in flow cytometry analysis. It is designed to specifically bind to its target, making it suitable for detecting target-expressing cells in flow cytometry.

2. What are the recommended storage conditions for the Trastuzumab antibody?

   A: The recommended storage condition for the Trastuzumab antibody (TP-083CL) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.

3. Can the Trastuzumab antibody be used in Western blotting?

   A: Yes, the Trastuzumab antibody (TP-083CL) can be used in Western blotting. It provides specific binding to its target, allowing for reliable detection in Western blot assays.

4. Is the Trastuzumab antibody effective in ELISA applications?

   A: Yes, the Trastuzumab antibody (TP-083CL) is effective in ELISA applications. It has been validated for use in such assays and provides reliable detection of its target.

5. What is the optimal dilution for using the Trastuzumab antibody in immunofluorescence?

   A: The optimal dilution for using the Trastuzumab antibody (TP-083CL) in immunofluorescence is typically 1:100 to 1:500. It is advisable to perform a dilution series to determine the best working concentration for your specific experimental conditions.

View the frequently asked questions answered by Creative Biolabs Support.

Citations

1. Chen, Wu, et al. "Performance of optimized wide pore superficially porous particles for separation of proteins and immunoglobulin G antibodies." Journal of Chromatographic Science 56.5 (2018): 416-424. https://doi.org/10.1093/chromsci/bmy016
   This research investigates the chromatographic performance of wide pore superficially porous particles (SPPs) with 3.5 μm particle size and 450 Å pore size for the separation of proteins and immunoglobulin G antibodies. The study compares different stationary phases (C4, SB-C18, and Diphenyl), examines the effects of temperature, column carryover, and chemical lifetime, and evaluates performance against other wide pore SPPs and sub-2 μm totally porous particles. The results demonstrate that SPPs with larger pore sizes provide superior chromatographic performance, particularly for large biomolecules, offering better resolution, sharper peaks, and reduced back pressure while maintaining excellent chemical stability at elevated temperatures.
   Creative Biolabs provided the humanized recombinant trastuzumab IgG1 intact antibody, which was used as a sample probe for evaluating the separation performance of different SPP columns. This contribution was significant as it allowed the researchers to demonstrate the advantages of larger pore SPPs (450 Å) in separating complex therapeutic antibodies, highlighting the practical application of these optimized particles in biopharmaceutical analysis and quality control processes.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TP-083CL, RRID: AB_3112050)

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Publications

Our products have been used extensively by scientists all over the world to accelerate their life science research. Here, we present a collection of publications that cite this product.

Chen, Wu, et al. "Performance of Optimized Wide Pore Superficially Porous Particles for Separation of Proteins and Immunoglobulin G Antibodies." Journal of chromatographic science 56.5 (2018): 416-424.

Biosimilar Overview

Please refer to Trastuzumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Trastuzumab.

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

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